Study on the Protective Effect of Hydrogen on Humid Infected Lung Health Workers

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In recent years, the impact of environmental fine particulate matter (PM2.5) on the health of the population has received widespread public attention. A large number of studies have confirmed the harm of PM2.5 exposure to the respiratory tract, but its mechanism is not yet fully understood. It is generally believed that it is mainly related to oxidative stress, inflammatory injury, immunotoxicity and gene polymorphism, and oxidative stress-induced lung injury is one of the hotspots in recent years.

In 2007, Ohsawa et al. reported that the hydrogen had a selective antioxidant function for the first time. Subsequently, the researchers found that hydrogen had selective antioxidant activity in the liver, kidney, heart, brain and diabetes, obesity, Anti-inflammatory ability. In recent years, with the realization of hydrogen inhalation therapy, its impact on lung disease was gradually concerned about the basic research has confirmed that hydrogen in lung ischemia and reperfusion, acute lung injury, lung injury, lung transplantation and other animal disease model In the role of protection [6], but its clinical intervention on the respiratory system at home and abroad has not been reported.

Sanitation workers as haze high exposure of people, PM2.5 impact on its health urgent need to pay attention. In this study, a randomized, double-blind, controlled study was conducted to investigate the protective effects of hydrogen and oxygen inhalation on the sanitation workers exposed to haze.

Objects and methods


From January to February in 2016, 107 healthy non-smoking sanitation workers in Shijiazhuang city center were selected as the research object, and the daily exposure period was 4 to 6 hours and prolonged for 30 days. Ten of these subjects were excluded because they were unable to cooperate with pulmonary function and exhaled nitric oxide (FeNO). Lost one. A total of 96 subjects were valid. All subjects were within 2 km radius of the study center. According to the order of grouping, odd number for the test group, even for the control group. (68 ± 10) kg, height 146 ~ 178 cm, mean (162 ± 178), average (46 ± 10) kg, average (47 ± 7) years old, weight 49.5 ~ 94.9 kg, average (68 ± 10) kg, height 146 ~ 178 cm, average (162 (47 ± 6) years old, weighing 47.0 ~ 105.8 kg, the average (66 ± 11) kg, the height of 146 ~ 176 years, the average age of 46 ~ Cm, mean (162 ± 7) cm. There was no significant difference in gender, age, height and weight between the two groups (P> 0.05). This study was approved by the Ethics Committee of the Second Hospital of Hebei Medical University (batch number: 2016002), and all subjects were given informed consent before enrollment.

Treatment method


According to the research group homemade form unified investigation of demographic information, daily working hours, symptoms and adverse reactions after intervention. 30 days after the end of the trial telephone follow-up of the above situation. Before the test, the investigators were trained in a unified group.

FeNO determination

Pulmonary function test:

(FVC), first-second forced lung capacity (FEV1), maximum expiratory volume (FEV1), maximum expiratory volume (FEV1), maximum expiratory volume (FEV1), maximum expiratory volume (FEV1), maximum expiratory volume (MMEF), maximum expiratory flow rate (PEFR), one second rate (FEV1 / FVC%) total of 5 ventilation function indicators, using the measured value of the measured value as a percentage of the estimated value for statistical analysis.

Cytokines and oxidative stress related products

All the subjects were collected venous blood and ultrasonic atomization induced sputum at 0, 8, 15 and 30 days after the experiment. Intravenous blood collection was performed in a vacuum blood collection tube (≥3 ml), centrifuged at 3 000 r / min in a 15 ml centrifuge tube, centrifuged at a centrifugal radius of 13.5 cm, centrifuged at low temperature for 10 min, and the supernatant was dispensed into (1.8%, 2.7%) for the hypertonic saline spit, the whole sputum during the sputum should be placed on the ice bag, repeat the process until the collection of sufficient sputum (≥3 ml). Add 3 times the volume of 10 g / L dithiothreitol, shake mix, placed in 37 ℃ water bath for 10 min, vortex 5 min, the 48 μm nylon mesh filter to 15 ml centrifuge tube 2 000 r / min centrifugation, centrifugal radius of 13.5 cm, low temperature centrifugation 10 min, the supernatant was dispensed into the EP tube, each tube 1.5 ml. After collection of serum and sputum supernatant were frozen at -80 ℃ refrigerator. IL-2, IL-10, transforming growth factor (TGF) -β1 (Anogen, Canada), human extracellular superoxide dismutase 3 (IL-2) (MMP-12, American AnaSpec), C-reactive protein (CRP, LDN, Germany) were measured by ELISA double-resistance sandwich method. All samples were tested at Shanghai Bio TNT Biotech Co., Ltd. All operations were performed by the laboratory technician in accordance with the kit instructions.

Statistical processing

The data were sorted and entered by two researchers. General survey data, pulmonary function and FeNO values were recorded in SAS 9.4. Demographic data such as age, height, and weight were described by ± s. Pulmonary function and FeNO were analyzed by covariance analysis to correct the initial factors. Cytokines and oxidative stress products were recorded in the SPSS 21.0 system. The Wilcoxon sign test was performed using the repeated measurements and the baseline values, and the factor levels were analyzed using the median (P25, P75) for general descriptive analysis. Subjective symptoms improved between groups using chi-square test. P <0.05 for the difference was statistically significant.


Atmospheric particles

All PM2.5 data are derived from the Shijiazhuang City, Hebei Province Environmental Protection Bureau to provide the subject of work to monitor the day the average air quality. In the “GB3095-2012 ambient air quality standards” in the provisions of PM2.5 ≥ 75 μg / m3 to determine the haze day. During the test period, the average pollution concentration of PM2.5 was 82.4 μg / m3, and the number of haze days was 11 days (36.7%), mainly in the pre-study period (64%), PM2.5 pollution level fluctuation.

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